Applications

Whole genome sequencing

Get the whole picture with fast results and detailed reports. Submit purified DNA to get results fast.

Data and deliverables

  • Assembled genome (.fasta)
  • Data quality report (.html)
  • Coverage plot (.png)
  • Read length histogram (.png)
  • Raw sequencing reads (.fastq)
  • Per-base sequencing information (.txt)
  • Responsive customer support
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Pricing

All prices shown are Canadian dollars and subject to local sales tax.

Service Type Data Output Target DNA Concentration Minimum Volume Purity (A260/280) Cost per Sample Target Turnaround Time
Bacterial WGS - Standard 200 Mb ≥ 50 ng/µL 20 µL 1.8 $135 2-3 Business Days
Bacterial WGS - Deep 350 Mb ≥ 50 ng/µL 20 µL $155

Frequently Asked Questions

What organisms can you sequence?

Our WGS service is optimized for bacterial whole genome sequencing. For yeast, fungi, or higher eukaryotes, please reach out through Discovery Mode and we can quote a custom project.

Should I choose Standard or Deep coverage?

Standard (200 Mb) delivers approximately at least 30× coverage for an average bacterial genome (1-7 Mb), which is sufficient for high-quality assembly and variant calling in most cases.

Deep (350 Mb) delivers approximately 30× coverage and is recommended when you have larger genomes (7-12 Mb), or your require higher sequencing depth for your analysis.

How should I prepare my DNA?

Submit purified genomic DNA at ≥ 50 ng/µL in at least 20 µL, with A260/280 of 1.8. Higher molecular weight input produces longer reads and better assemblies (>50 % of bases above 10 Kb).

What files will I receive?

By default, you will receive a consensus sequence (.fasta), annotations (multiple formats), .fastq files, and quality control files (.png, .html). If you require custom analysis, please specify in the “Additional Information” section.

When will I receive my data?

WGS projects complete in 2–3 business days from receipt of samples, depending on throughput tier. If you need data ASAP, let us know.

Still have questions? Email us at support@flowgenomics.com