PrepLESS Flow lets you skip the miniprep entirely. Submit colonies circled on an agar plate, resuspended colonies in water, or 100 µL of liquid culture, and we’ll handle plasmid extraction and sequencing in one workflow. It’s $30 per sample and delivers in 1 business day — ideal for cloning workflows where the miniprep step is the bottleneck.

From small plasmids (~1 kb) up to 300 kb constructs. The size of your plasmid determines which service tier to choose: Standard for <25 kb, Large for 25–125 kb, and Extra-large for 125–300 kb. See the pricing tables above for concentration and volume requirements at each tier.

Submit column-purified or bead-purified plasmid DNA at the concentration listed for your service tier. Avoid linearization unless you specifically want linear-read coverage — our default workflow produces full-length reads from supercoiled or relaxed circular input. For PrepLESS Flow, just send colonies or culture and we will handle the rest.

By default, you will receive a single consensus sequence (.fasta), simulated agarose gel (.png), chromatogram (.ab1), annotated plasmid map (.gbk, .html), data quality report (.html), coverage plot (.png), read length histogram (.png), sequencing reads (.fastq), and per-base sequencing information (.txt). If you would like to request additional files or analyses, please specify in the “Additional Information” section.

Standard plasmid sequencing and PrepLESS Flow complete in 1 business day from receipt of samples, although we typically measure return time in the hours scale. If you need data ASAP, let us know.